The dominant "stacking" approach to longevity fails due to cellular energy bottlenecks. This paper establishes a three-phase "Energy-First" protocol: NAD+ restoration, followed by autophagy induction, followed by senolytic clearance. The sequence succeeds because aged cells lack the energy reserves to execute multiple repair processes simultaneously.
How sequential dosing resolves rapamycin's contradictions for muscle and immune function. The mTOR inhibitor that suppresses protein synthesis and immunity paradoxically improves both—when administered to prepared tissues following the Integration Protocol.
Senolytics kill senescent cells—but killing is only half the problem. Why unprepared tissues experience inflammatory overload while prepared tissues achieve genuine rejuvenation. The debris clearance bottleneck and how sequential preparation transforms outcomes.
The twelve-week Integration Protocol prepares tissues for advanced interventions. Phase 4 introduces stem cell therapy into terrain cleared of senescent cells and restored to energetic competence. Phase 5 explores partial epigenetic reprogramming through transient Yamanaka factor expression. Why unprepared tissue is hostile to engraftment—and how sequential preparation transforms regenerative medicine outcomes.
In Review